CoraLite® Plus 647-conjugated Phospho-IkB Beta (Thr19/Ser23) Recombinant monoclonal antibody

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  FC experiment of HT-29 using CL647-84403

  1X10^6 HT-29 cells untreated (dashed lines) or treated with TNF alpha and Calyculin A which intracellularly stained with 0.13 ug Coralite® Plus 647 Phospho-Ikb Beta (Thr19/Ser23) Recombinant Antibody (CL647-84403, Clone:241061B2)(red), or 0.13 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-IkB Beta (Thr19/Ser23) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-84403

Clone No.241061B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

p IkB Beta, p IκB β, pho IkB Beta, pho-IkB Beta, Phos-IkB Beta

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Tested Applications

• Positive FC (Intra) detected in: TNF alpha and Calyculin A treated HT-29 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-84403 targets Phospho-IkB Beta (Thr19/Ser23) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• Calculated Molecular Weight: 356 aa, 38 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC015528
• Gene Symbol: IkB Beta
• Gene ID (NCBI): 4793
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15653
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23.