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Phospho-JNK (Thr183) Recombinant monoclonal antibody, PBS Only (80435-8-PBS)

Phospho-JNK (Thr183) Recombinant monoclonal antibody, PBS Only

Name: Phospho-JNK (Thr183) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 80435-8-PBS-100UG

Phospho-JNK (Thr183) Uni-rAb^® Recombinant Antibody for WB, Dot Blot, Indirect ELISA

Cat No. 80435-8-PBS

Clone No.253546C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Dot Blot, Indirect ELISA

Synonyms

JNK (Thr183), Phospho-JNK, phospho-SAPK, phospho-SAPK (Thr183), Phospho-SAPK/JNK

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBDot Blot

WB analysis using 80435-8-RR (same clone as 80435-8-PBS)
Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 80435-8-RR (same clone as 80435-8-PBS)
Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.
Dot Blot experiment of / using 80435-8-RR (same clone as 80435-8-PBS)
Dot blot analysis was used to confirm the specificity of 80435-8-RR,Phospho-SAPK/JNK (Thr183) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

80435-8-PBS targets Phospho-JNK (Thr183) in WB, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	mitogen-activated protein kinase 8
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	42 kDa, 50 kDa
GenBank Accession Number	NM_138982
Gene Symbol	JNK
Gene ID (NCBI)	5599
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P45983
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. Phospho-JNK (Thr183) is the activated form of c-Jun N-terminal kinase (JNK), a MAPK family serine/threonine kinase. Phosphorylation at Thr183 (often with Tyr185) by MKK4/7 activates JNK, regulating cell apoptosis, proliferation and stress responses. (PMID: 40538844)