Phospho-KAP1 (Ser824) Recombinant monoclonal antibody

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  WB analysis using 87309-1-RR

  Non-treated NIH/3T3 cells and etoposide treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87309-1-RR (Phospho-KAP1 (Ser824) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  IF Staining of NIH/3T3 using 87309-1-RR

  Immunofluorescent analysis of (4% PFA) fixed etoposide treated NIH/3T3 cells using Phospho-KAP1 (Ser824) antibody (87309-1-RR, Clone: 252561H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of NIH/3T3 using 87309-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Phospho-KAP1 (Ser824) antibody (87309-1-RR, Clone: 252561H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-KAP1 (Ser824) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87309-1-RR

Clone No.252561H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Phospho KAP1 (Ser824), Phospho-KAP1, Phospho-KAP-1, Phospho-KAP-1 (Ser824), Phospho-KAP1(Ser824)

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Tested Applications

• Positive WB detected in: etoposide treated NIH/3T3 cells
• Positive IF/ICC detected in: NIH/3T3 cells, etoposide treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87309-1-RR targets Phospho-KAP1 (Ser824) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tripartite motif-containing 28
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_005762
• Gene Symbol: KAP1
• Gene ID (NCBI): 10155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13263
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. Phospho-KAP1 (Ser824) refers to the phosphorylated form of KAP1 (KRAB-associated protein 1) at Serine 824. This post-translational modification plays a significant role in the regulation of gene expression and chromatin structure.