Phospho-MNK1 (Thr250/255) Recombinant antibody

Phospho-MNK1 (Thr250/255) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4P9

Cat no : 81398-1-RR

Synonyms

MKNK1, MNK1, Phospho-MKNK1 (Thr250/255), Phospho-MNK1 (Thr250/255)

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Validation Data Gallery

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  WB analysis of HEK-293T using 81398-1-RR

  Non-treated HEK-293T cells, phosphatase inhibitor and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81398-1-RR (Phospho-MNK1 (Thr250/255) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: HEK-293T cells, λ phosphatase treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81398-1-RR targets Phospho-MNK1 (Thr250/255) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: MAP kinase interacting serine/threonine kinase 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC002755
• Gene Symbol: MKNK1
• Gene ID (NCBI): 8569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MNK1/MKNK1 (MAPK Interacting Serine/Threonine Kinase 1) is a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. MNK1 regulates diverse biologic processes including translation, cell proliferation, and differentiation through phosphorylation of different substrates(PMID: 29045529). Through phosphorylation MNK1 regulates the function of eukaryotic translation initiation factor 4E (eIF4E), a key player in translational control, whose expression is mostly upregulated in cancer patients.