Phospho-MYPT1 (Thr853) Recombinant monoclonal antibody

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  WB analysis using 87088-1-RR

  HeLa cells, HEK-293 cells and C6 cells were subjected to SDS PAGE followed by western blot with 87088-1-RR (Phospho-MYPT1 (Thr853) antibody) blocked with BSA only, Phospho-MYPT1 (Thr853) peptide or non-Phospho peptide at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 antibody (66095-1-Ig) as loading control.

Phospho-MYPT1 (Thr853) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87088-1-RR

Clone No.252239E11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

MYPT1 (Thr853), Phospho MYPT1 (Thr853), Phospho-MYPT1, Phospho-MYPT1-Thr853, p-MYPT1 (T853)

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87088-1-RR targets Phospho-MYPT1 (Thr853) in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Calculated Molecular Weight: 1030 aa, 115 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC111752
• Gene Symbol: MYPT1
• Gene ID (NCBI): 4659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14974
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Phospho-MYPT1 (Thr853) is a phosphorylated form of MYPT1 (myosin phosphatase target subunit 1) at threonine 853. This phosphorylation event is associated with the regulation of smooth muscle contraction and cell motility. The Rho-associated protein kinase (ROCK) is known to phosphorylate MYPT1 at Thr853, which in turn suppresses the activity of myosin light chain phosphatase (MLCP), leading to an increase in myosin light chain phosphorylation and subsequent smooth muscle contraction. (PMID: 24712327)