Phospho-MYPT1 (Thr853) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87088-1-RR (same clone as 87088-1-PBS)

  HeLa cells, HEK-293 cells and C6 cells were subjected to SDS PAGE followed by western blot with 87088-1-RR (Phospho-MYPT1 (Thr853) antibody) blocked with BSA only, Phospho-MYPT1 (Thr853) peptide or non-Phospho peptide at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 87088-1-PBS in a different storage buffer formulation.

Phospho-MYPT1 (Thr853) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87088-1-PBS

Clone No.252239E11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

MYPT1 (Thr853), Phospho MYPT1 (Thr853), Phospho-MYPT1, Phospho-MYPT1-Thr853, p-MYPT1 (T853)

---

Product Information

87088-1-PBS targets Phospho-MYPT1 (Thr853) in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Calculated Molecular Weight: 1030 aa, 115 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC111752
• Gene Symbol: MYPT1
• Gene ID (NCBI): 4659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14974
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Phospho-MYPT1 (Thr853) is a phosphorylated form of MYPT1 (myosin phosphatase target subunit 1) at threonine 853. This phosphorylation event is associated with the regulation of smooth muscle contraction and cell motility. The Rho-associated protein kinase (ROCK) is known to phosphorylate MYPT1 at Thr853, which in turn suppresses the activity of myosin light chain phosphatase (MLCP), leading to an increase in myosin light chain phosphorylation and subsequent smooth muscle contraction. (PMID: 24712327)