Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody, PBS Only

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  WB analysis of HEK-293 using 85804-1-RR (same clone as 85804-1-PBS)

  Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 85804-1-RR (same clone as 85804-1-PBS)

  Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 85804-1-RR (same clone as 85804-1-PBS)

  Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85804-1-PBS

Clone No.250098F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

P Myosin Light Chain 2 (Thr18/Ser19), Phos Myosin Light Chain 2 (Thr18/Ser19), Phos-Myosin Light Chain 2 (Thr18/Ser19), Phospho-MYL12A (Thr18/Ser19), Phospho-MYL12B

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Product Information

85804-1-PBS targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, light chain 12A, regulatory, non-sarcomeric
• Calculated Molecular Weight: 171 aa, 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC016372
• Gene Symbol: MYL12A
• Gene ID (NCBI): 10627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19105
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.