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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombi (85804-1-RR)

    WB analysis of HEK-293 using 85804-1-RR

    Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombi (85804-1-RR)

    WB analysis of NIH/3T3 using 85804-1-RR

    Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombi (85804-1-RR)

    WB analysis of HSC-T6 using 85804-1-RR

    Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85804-1-RR
Clone No.250098F12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

P Myosin Light Chain 2 (Thr18/Ser19), Phos Myosin Light Chain 2 (Thr18/Ser19), Phos-Myosin Light Chain 2 (Thr18/Ser19), Phospho-MYL12A (Thr18/Ser19), Phospho-MYL12B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HEK-293 cells, Calyculin A treated HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85804-1-RR targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name myosin, light chain 12A, regulatory, non-sarcomeric
Calculated Molecular Weight 171 aa, 20 kDa
Observed Molecular Weight 19-20 kDa
GenBank Accession NumberBC016372
Gene Symbol MYL12A
Gene ID (NCBI) 10627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19105
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.

Protocols

Product Specific Protocols
WB protocol for Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody 85804-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols