CoraLite® Plus 647-conjugated Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant monoclonal antibody (CL647-85804)

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-85804 Copied!

Clone No. 250098F12

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

MYL12B, MYL9, Myosin Light Chain 2 (Thr18/Ser19), P MYL12A, P MYL12B

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  FC experiment of NIH/3T3 using CL647-85804

  1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.2 ug Coralite® Plus 647 Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant Antibody (CL647-85804, Clone:250098F12)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: Calyculin A treated NIH/3T3 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-85804 targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin, light chain 12A, regulatory, non-sarcomeric
• Calculated Molecular Weight: 171 aa, 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC016372
• Gene Symbol: MYL12A
• Gene ID (NCBI): 10627
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19105
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.