CoraLite® Plus 488-conjugated Phospho-P53 (Ser46) Monoclonal antibody
Phospho-P53 (Ser46) Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D10A12
Cat no : CL488-67900
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | UV treated HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Sample-dependent, check data in validation data gallery |
Product Information
CL488-67900 targets Phospho-P53 (Ser46) in FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | tumor protein p53 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 53 kDa |
GenBank accession number | BC003596 |
Gene symbol | TP53 |
Gene ID (NCBI) | 7157 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)