Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Recombinant monoclonal antibody, PBS Only

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• Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Recombinant monoclonal antibody, PBS Only (87648-2-PBS)

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  WB analysis using 87648-2-RR (same clone as 87648-2-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87648-2-RR (Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87648-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 87648-2-RR (same clone as 87648-2-PBS)

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87648-2-RR (Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87648-2-PBS in a different storage buffer formulation.

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87648-2-PBS

Clone No.253138E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

PAK4 (Ser474), PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560), Phospho-PAK4, Phospho-PAK4 (Ser474), Phospho-PAK5 (Ser602)

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Product Information

87648-2-PBS targets Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: p21 protein (Cdc42/Rac)-activated kinase 4
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_001014831
• Gene Symbol: PAK4
• Gene ID (NCBI): 10298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O96013
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily. Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) refers to the phosphorylated forms of the Group II p21-Activated Kinases (PAKs) at specific serine residues in their kinase activation loops. These phosphorylation events are essential for the catalytic activation of Group II PAKs and serve as definitive markers of their enzymatic activity. (PMID: 17292838)