Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Recombinant monoclonal antibody

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• Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Recombinant monoclonal antibody (87648-2-RR)

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  WB analysis using 87648-2-RR

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87648-2-RR (Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

• 

  WB analysis using 87648-2-RR

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87648-2-RR (Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87648-2-RR

Clone No.253138E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PAK4 (Ser474), PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560), Phospho-PAK4, Phospho-PAK4 (Ser474), Phospho-PAK5 (Ser602)

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, λ phosphatase treated HeLa cells, λ phosphatase treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87648-2-RR targets Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: p21 protein (Cdc42/Rac)-activated kinase 4
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_001014831
• Gene Symbol: PAK4
• Gene ID (NCBI): 10298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O96013
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily. Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) refers to the phosphorylated forms of the Group II p21-Activated Kinases (PAKs) at specific serine residues in their kinase activation loops. These phosphorylation events are essential for the catalytic activation of Group II PAKs and serve as definitive markers of their enzymatic activity. (PMID: 17292838)