Tested Applications
| Positive WB detected in | NIH/3T3 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85223-3-RR targets Phospho-PDPK1 (Ser241) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | 3-phosphoinositide dependent protein kinase-1 |
| Calculated Molecular Weight | 556 aa, 63 kDa |
| Observed Molecular Weight | 58-68 kDa |
| GenBank Accession Number | BC012103 |
| Gene Symbol | PDPK1 |
| Gene ID (NCBI) | 5170 |
| RRID | AB_3744115 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RR | Download protocol |
| IF protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RR | Download protocol |
| WB protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-PDPK1 (Ser241) recombinant monoclonal antibody (Cat# 85223-3-RR) has 3 citations. These appear in Cancer Letters, Journal of Translational Medicine, and Pathology Research and Practice. The associated research fields include pancreatic ductal adenocarcinoma (PDAC) transendothelial migration, hepatocellular carcinoma oncogenic signaling via AKT activation, and gastric ulcer protection through the PI3K/AKT pathway. All three studies utilized the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cancer Lett Tumor-derived CDHR2-enriched extracellular vesicles engage ANPEP on liver sinusoidal endothelial cells to facilitate transendothelial migration in PDAC. | ||
J Transl Med Identification of the CYP19A1-GPER1 axis as a critical oncogenic driver in hepatocellular carcinoma via AKT activation. | ||
Pathol Res Pract Identification of bioactive components in Wuji Wan and their protective effects against gastric ulcer via regulating PIK3CA-mediated PI3K/AKT pathway. |
