CoraLite® Plus 488-conjugated Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody (CL488-85223-3)

Phospho-PDPK1 (Ser241) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-85223-3 Copied!

Clone No. 250623A11

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

p PDPK1, p PDPK1 (Ser241), PDPK1 (Ser241), Phospho PDPK1 (Ser241), Phospho-PDPK1

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  FC experiment of NIH/3T3 using CL488-85223-3

  1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Coralite® Plus 488 Phospho-Pdpk1 (Ser241) Recombinant Antibody (CL488-85223-3, Clone:250623A11)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: λ phosphatase treated NIH/3T3 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85223-3 targets Phospho-PDPK1 (Ser241) in FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: 3-phosphoinositide dependent protein kinase-1
• Calculated Molecular Weight: 556 aa, 63 kDa
• Observed Molecular Weight: 58-68 kDa
• GenBank Accession Number: BC012103
• Gene Symbol: PDPK1
• Gene ID (NCBI): 5170
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15530
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.