Phospho-PKC Alpha (Ser657) Polyclonal antibody

Phospho-PKC Alpha (Ser657) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28926-1-AP

Synonyms

AAG6, Phospho-PKC Alpha (Ser657), PKC A, PKC alpha, PKCA, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha



Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

28926-1-AP targets Phospho-PKC Alpha (Ser657) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein kinase C, alpha
Calculated Molecular Weight 77 kDa
Observed Molecular Weight77 kDa
GenBank Accession NumberAK055431
Gene Symbol PRKCA
Gene ID (NCBI) 5578
RRIDAB_2918214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

Protocols

Product Specific Protocols
WB protocol for Phospho-PKC Alpha (Ser657) antibody 28926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Neurokinin-1 receptor drives PKCɑ-AURKA/N-Myc signaling to facilitate the neuroendocrine progression of prostate cancer

Authors - Xiao-Wei Zhang
mouseWB

Appl Biochem Biotechnol

Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC-α

Authors - Lichao Zhu