Phospho-PLA2G4A (Ser505) Polyclonal antibody

Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 28925-1-AP

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, Phospho-PLA2G4A (Ser505), PLA2G4, PLA2G4A

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Validation Data Gallery

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  WB analysis using 28925-1-AP

  Non-treated HeLa and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28925-1-AP (Phospo-PLA2G4A (Ser505) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

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  IF Staining of HeLa using 28925-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PLA2G4A (Ser505) antibody (28925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: λ phosphatase treated HeLa cells
• Positive IF detected in: λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28925-1-AP targets Phospho-PLA2G4A (Ser505) in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase A2, group IVA (cytosolic, calcium-dependent)
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC114340
• Gene Symbol: PLA2G4A
• Gene ID (NCBI): 5321
• RRID: AB_2918213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and and 0.05%BSA.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)