Phospho-POLR2A (Ser2) Recombinant antibody

Phospho-POLR2A (Ser2) {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84974-1-RR
Clone No.241829B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

phospho-CTD, Phospho-POLR2A, Phospho-Rpb1 CTD, Phospho-Rpb1 CTD (Ser2), phospho-Ser2-CTD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293 cells, λ phosphatase treated HEK-293 cells
Positive FC (Intra) detected inλ phosphatase treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84974-1-RR targets Phospho-POLR2A (Ser2) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Calculated Molecular Weight 1970 aa, 217 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC137231
Gene Symbol POLR2A
Gene ID (NCBI) 5430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)

Protocols

Product Specific Protocols
WB protocol for Phospho-POLR2A (Ser2) antibody 84974-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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