Phospho-POLR2A (Ser2) Recombinant antibody

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  WB analysis using 84974-1-RR

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 84974-1-RR (Phospho-POLR2A (Ser2) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

• 

  FC experiment of HEK-293 using 84974-1-RR

  1x10^6 λ phosphatase treated HEK-293 cells were intracellularly stained with 0.06 ug Phospho-POLR2A (Ser2) Recombinant antibody (84974-1-RR, Clone:241829B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-POLR2A (Ser2) Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84974-1-RR

Clone No.241829B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

phospho-CTD, Phospho-POLR2A, Phospho-Rpb1 CTD, Phospho-Rpb1 CTD (Ser2), phospho-Ser2-CTD

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Tested Applications

• Positive WB detected in: HEK-293 cells, λ phosphatase treated HEK-293 cells
• Positive FC (Intra) detected in: λ phosphatase treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84974-1-RR targets Phospho-POLR2A (Ser2) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 1970 aa, 217 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC137231
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P24928
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)