CoraLite® Plus 647-conjugated Phospho-POLR2A (Ser2) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-POLR2A (Ser2) Recombinant monoclonal antibody (CL647-84974)

Phospho-POLR2A (Ser2) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-84974 Copied!

Clone No. 241829B4

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

phospho-CTD, Phospho-POLR2A, Phospho-Rpb1 CTD, Phospho-Rpb1 CTD (Ser2), phospho-Ser2-CTD

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  FC experiment of HEK-293T using CL647-84974

  1X10^6 HEK-293T cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Polr2a (Ser2) Recombinant Antibody (CL647-84974, Clone:241829B4)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: λ phosphatase treated HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-84974 targets Phospho-POLR2A (Ser2) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 1970 aa, 217 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC137231
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24928
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)