Phospho-POLR2A (Ser7) Recombinant antibody, PBS Only

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  WB analysis using 83932-1-RR (same clone as 83932-1-PBS)

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 83932-1-RR (POLR2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83932-1-PBS in a different storage buffer formulation.

Phospho-POLR2A (Ser7) {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83932-1-PBS

Clone No.240829F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

POLR2A, EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A

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Product Information

83932-1-PBS targets Phospho-POLR2A (Ser7) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_000937
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P24928
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.