Phospho-POLR2A (Ser7) Recombinant monoclonal antibody

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• Phospho-POLR2A (Ser7) Recombinant monoclonal antibody (83932-1-RR)

• 

  WB analysis using 83932-1-RR

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 83932-1-RR (POLR2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  ChIP experiment of HeLa using 83932-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-POLR2A (Ser7) (83932-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Phospho-POLR2A (Ser7) Uni-rAb^® Recombinant Antibody for WB, ELISA, ChIP-qPCR

Cat No. 83932-1-RR

Clone No.240829F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA, ChIP-qPCR

Synonyms

p POLR2A, p POLR2A (Ser7), p POLR2A Ser7, Phospho POLR2A (Ser7), Phospho POLR2A Ser7

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83932-1-RR targets Phospho-POLR2A (Ser7) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_000937
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• RRID: AB_3671513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P24928
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.