Tested Applications
| Positive FC (Intra) detected in | λ phosphatase treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-81475 targets Phospho-RPS6KA1 (Thr359/Ser363) in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
| Calculated Molecular Weight | 735 aa, 83 kDa |
| Observed Molecular Weight | 83-90 kDa |
| GenBank Accession Number | BC014966 |
| Gene Symbol | RPS6KA1 |
| Gene ID (NCBI) | 6195 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15418 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)
