Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody

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• Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody (87071-2-RR)

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 87071-2-RR Copied!

Clone No. 252281E7

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Synonyms

RPS6, S6, 40S ribosomal protein S6, OK/SW-cl.2, Phosphoprotein NP33

• 

  WB analysis of MCF-7 using 87071-2-RR

  Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87071-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  SEC-HPLC of 87071-2-RR

  The purity of Human Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody (Cat. No. 87071-2-RR) was greater than 95% as determined by SEC-HPLC.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human pancreas using 87071-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded λ phosphatase treated human pancreas tissue using Phospho-S6 Ribosomal protein (Ser235/236) antibody (87071-2-RR, Clone: 252281E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human pancreas using 87071-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using Phospho-S6 Ribosomal protein (Ser235/236) antibody (87071-2-RR, Clone: 252281E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: IGF-1 treated MCF-7 cells
• Positive IF-P detected in: human pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87071-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Mitogens also stimulate p90 ribosomal S6 kinases (RSK1-4), which can phosphorylate the same Ser235/236 residues independently of mTOR, providing a parallel pathway that links MAPK activation to translational control. (PMID: 17360704)