Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody

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  WB analysis of HEK-293 using 81184-1-RR

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81184-1-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

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  IF Staining of HeLa using 81184-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) antibody (81184-1-RR, Clone: 5J13 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HEK-293 using 81184-1-RR

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone:5J13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 81184-1-RR

Clone No.5J13

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

p RPS6, p RPS6 (Ser240/244), p S6 Ribosomal protein, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein Ser240/244

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293 cells
• Positive IF/ICC detected in: Calyculin A treated HeLa cells
• Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81184-1-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_001010
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• RRID: AB_2935425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.