Phospho-SMAD1 (Ser214) Polyclonal antibody

Phospho-SMAD1 (Ser214) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28895-1-AP

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, Phospho-SMAD1 (Ser214), SMAD 1, SMAD family member 1, SMAD1



Tested Applications

Positive WB detected inBMP2 treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Sample-dependent, check data in validation data gallery

Product Information

28895-1-AP targets Phospho-SMAD1 (Ser214) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC001878
Gene symbol SMAD1
Gene ID (NCBI) 4086
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)

Protocols

Product Specific Protocols
WB protocol for Phospho-SMAD1 (Ser214) antibody 28895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols