Tested Applications
| Positive WB detected in | L02 cells, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19561-1-AP targets Phospho-SPHK1 (Ser225) in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | sphingosine kinase 1 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 45-47 kDa |
| GenBank Accession Number | NM_182965 |
| Gene Symbol | SPHK1 |
| Gene ID (NCBI) | 8877 |
| RRID | AB_10950399 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-SPHK1 (Ser225) antibody 19561-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SPHK1 antibody (Cat# 19561-1-AP) has 24 citations in journals including Cancer Communications, Cell Death & Differentiation, Cell Death & Disease, Phytomedicine, J Transl Med, Phytother Res, Environ Pollut, J Ethnopharmacol, Ecotoxicol Environ Saf, Eur J Pharmacol, Int Immunopharmacol, BBA MBD, Sci Rep, Biomol Ther, Front Physiol, Arch Biochem Biophys, Exp Eye Res, Dig Dis Sci, Electromagn Biol Med, Oncotarget, and Mechanobiol Med. Research fields span oncology, rheumatoid arthritis, angiogenesis, sphingolipid metabolism, and liver disease.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis. | ||
Cell Death Dis Autophagy-dependent secretion of ENO1 mediates chemoresistance of glioblastoma and tumor microenvironment remodeling. | ||
Cell Death Dis POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling. | ||
Phytomedicine Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation. | ||
Phytomedicine Novel mechanism by which geniposide alleviates rheumatoid arthritis: Targeted inhibition of SphK1 negatively regulates S1P signaling, promotes HIF-1α acetylation-mediated degradation and blocks the VEGF-induced synovial angiogenesis. |
