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Phospho-SPHK1 (Ser225) Polyclonal antibody

Phospho-SPHK1 (Ser225) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19561-1-AP

Synonyms

Phospho-SPHK1 (Ser225), SK 1, sphingosine kinase 1, SPHK, SPHK1 phopho, SPHK1-Phospho-Ser225, SPK, SPK 1



Tested Applications

Positive WB detected inL02 cells, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

19561-1-AP targets Phospho-SPHK1 (Ser225) in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sphingosine kinase 1
Calculated molecular weight 51 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberNM_182965
Gene symbol SPHK1
Gene ID (NCBI) 8877
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).

Protocols

Product Specific Protocols
WB protocol for Phospho-SPHK1 (Ser225) antibody 19561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Imbalanced sphingolipid signaling is maintained as a core proponent of a cancerous phenotype in spite of metabolic pressure and epigenetic drift.

Authors - Monique M P Speirs
humanWB

Cell Death Dis

POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling.

Authors - Zhiyong Shen
WB

Arch Biochem Biophys

Cross talk between MMP2-Spm-Cer-S1P and ERK1/2 in proliferation of pulmonary artery smooth muscle cells under angiotensin II stimulation.

Authors - Animesh Chowdhury
  • KD Validated
ratWB

Phytother Res

Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.

Authors - Yan Wang
mouseWB

Biochim Biophys Acta Mol Basis Dis

Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy.

Authors - Federica Pierucci
humanWB

Ecotoxicol Environ Saf

A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway.

Authors - Liangjing Chen