Phospho-STAT1 (Ser727) Polyclonal antibody

Phospho-STAT1 (Ser727) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28977-1-AP

Synonyms

DKFZp686B04100, ISGF 3, Phospho-STAT1 (Ser727), STAT1, STAT91



Tested Applications

Positive WB detected inIFN gamma treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

28977-1-AP targets Phospho-STAT1 (Ser727) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight 83 kDa
Observed molecular weight84 kDa
GenBank accession numberBC002704
Gene symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant during lipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT1 (Ser727) antibody 28977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Res Sq

The RhoA GTPase regulates Type I Interferon Signaling in Systemic lupus erythematosus

Authors - Wei Fan
humanWB

Cancer Res Commun

Differential Regulation of the Stimulator of Interferon Genes pathway in Human Papillomavirus positive and negative Head and Neck Cancers

Authors - Emma Saulters

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-24-2022)

Works well for western blots. Only had a few unspecific bands but the antibody showed nice results for a phosphorylated protein.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human cell lines