Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinant monoclonal antibody

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• Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinant monoclonal antibody (80115-1-RR)

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 80115-1-RR Copied!

Clone No. 2P21

Publications (17)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

p STAT5A, p STAT5A (Tyr694), p STAT5A (Tyr694)/STAT5B (Tyr699), p STAT5A Tyr694, p STAT5A Tyr694/STAT5B Tyr699

• 

  WB analysis using 80115-1-RR

  Non-treated TF-1 and GM-CSF (HZ-1002) treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80115-1-RR (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of TF-1 using 80115-1-RR

  1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF which intracellularly stained with 0.06 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinant antibody (80115-1-RR, Clone:2P21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: GM-CSF treated TF-1 cells
• Positive FC (Intra) detected in: GM-CSF treated TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80115-1-RR targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: signal transducer and activator of transcription 5A
• Calculated Molecular Weight: 794 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC027036
• Gene Symbol: STAT5A
• Gene ID (NCBI): 6776
• RRID: AB_2918866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42229
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

Publications

What published studies show

The Rabbit mAb anti-STAT5 (80115-1-RR) has 17 citations across journals including Experimental Hematology & Oncology, Redox Biology, Journal of Experimental & Clinical Cancer Research, Cancer Letters, Cell Death Discovery, Oncogenesis, Frontiers in Immunology, and mBio. Research fields span acute myeloid leukemia, hepatocellular carcinoma, cervical cancer, colon cancer, inflammatory arthritis, ischemic stroke, viral infection, and mesenchymal stem cell biology, primarily using Western blot and IHC applications in human, mouse, and pig samples.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Exp Hematol Oncol; MMP14 from BM-MSCs facilitates progression and Ara-C resistance in acute myeloid leukemia via the JAK/STAT pathway; Authors - Jinxian Wu
human,mouse	WB	Redox Biol; Neutrophil extracellular traps exacerbate inflammatory arthritis by inhibiting γδ Treg cell differentiation via the AIM2 inflammasome.; Authors - Yipeng Zeng
mouse	WB	J Exp Clin Cancer Res; VSIG4+ tumor-associated macrophages mediate neutrophil infiltration and impair antigen-specific immunity in aggressive cancers through epigenetic regulation of SPP1; Authors - Zongfu Pan
mouse	WB	Carbohydr Polym; Structural characterizations and antiaging activities of hydrolyzed low-molecular-weight polysaccharides from Polygoni Multiflori Radix Praeparata; Authors - Wei Xu
mouse,human	WB,IHC	Cancer Lett; FLT3-Selective PROTAC: Enhanced Safety and Increased Synergy with Venetoclax in FLT3-ITD Mutated Acute Myeloid Leukemia; Authors - Yuxin Tan
	WB	J Immunother Cancer; Endosialin-positive CAFs promote hepatocellular carcinoma progression by suppressing CD8+ T cell infiltration; Authors - Lunbiao Gan