|Positive WB detected in||GM-CSF treated TF-1 cells|
|Positive FC detected in||GM-CSF treated TF-1 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Sample-dependent, check data in validation data gallery|
80115-1-RR targets Phospho-STAT5A (Tyr694) in WB, FC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||signal transducer and activator of transcription 5A|
|Calculated molecular weight||794 aa, 92 kDa|
|Observed molecular weight||90-95 kDa|
|GenBank accession number||BC027036|
|Gene ID (NCBI)||6776|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.