FITC-conjugated Phospho-STAT5A (Tyr694) Recombinant antibody

Phospho-STAT5A (Tyr694) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

FITC Fluorescent Dye

CloneNo.

2P21

Cat no : FITC-80115

Synonyms

MGF, STAT5, STAT5A



Tested Applications

Positive FC detected inGM-CSF treated TF-1 cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

FITC-80115 targets Phospho-STAT5A (Tyr694) in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight90-95 kDa
GenBank accession numberBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.