Phospho-TAK1 (Ser439) Recombinant monoclonal antibody

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  WB analysis of HEK-293 using 87129-2-RR

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 87129-2-RR (Phospho-TAK1 (Ser439) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-TAK1 (Ser439) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87129-2-RR

Clone No.252365A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho TAK1 (Ser412), Phospho-TAK1, Phospho-TAK1 (Ser412), Phospho-TAK1(Ser412), Phospho-TAK1-Ser412

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Tested Applications

• Positive WB detected in: HEK-293 cells, λ phosphatase treated HEK-293 cells

This antibody is equivalent to Ser412 in mice.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87129-2-RR targets Phospho-TAK1 (Ser439) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase 7
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: NM_145331
• Gene Symbol: TAK1
• Gene ID (NCBI): 6885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43318
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. Phospho-TAK1 (Ser439) refers to the phosphorylated form of TAK1 at serine residue 439. TAK1, also known as Transforming Growth Factor-beta activated kinase 1, is a key kinase involved in the regulation of various signaling pathways, including those related to inflammation, immune responses, and cell survival. The phosphorylation of TAK1 at Ser439 is an important post-translational modification that can affect its activity and interaction with other proteins within these pathways. This phosphorylation event is crucial for TAK1's role in signal transduction and its involvement in cellular processes such as the activation of NF-κB and MAPK pathways. It plays a significant role in the activation of downstream signaling cascades that regulate various cellular processes, including cell growth, proliferation, and survival. This antibody is equivalent to Ser412 in mice.