Phospho-TAU (Ser202) Recombinant monoclonal antibody

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  WB analysis using 86748-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86748-1-RR (Phospho-TAU (Ser202) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. The FAD3T mouse is a triple transgenic model expressing APP, PS1, and Tau transgenes.

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  WB analysis using 86748-1-RR

  Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 86748-1-RR (Phospho-TAU (Ser202) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

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  IF Staining of mouse brain using 86748-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-TAU (Ser202) antibody (86748-1-RR, Clone: 251172C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Phospho-TAU (Ser202) Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 86748-1-RR

Clone No.251172C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

p TAU, p TAU (Ser202), p TAU Ser202, phos TAU, Phospho TAU

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Tested Applications

• Positive WB detected in: FAD3T mouse brain tissue, mouse brain tissue, λ phosphatase treated mouse brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86748-1-RR targets Phospho-TAU (Ser202) in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.