Phospho-ULK1 (Ser758) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82776-8-RR (same clone as 82776-8-PBS)

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Alpha Tubulin antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 82776-8-PBS in a different storage buffer formulation.

• 

  WB analysis using 82776-8-RR (same clone as 82776-8-PBS)

  Non-treated, phosphatase inhibitor treated and λ phosphatase treated PC-3 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Vinculin antibody (66305-1-Ig) as loading control. This data was developed using the same antibody clone with 82776-8-PBS in a different storage buffer formulation.

Phospho-ULK1 (Ser758) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 82776-8-PBS

Clone No.240770A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

P ULK1 (Ser758), Phos ULK1 (Ser758), Phospho-ULK1 (Ser757), Phos-ULK1 (Ser758), P-ULK1 (Ser758)

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Product Information

82776-8-PBS targets Phospho-ULK1 (Ser758) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: unc-51-like kinase 1 (C. elegans)
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75385
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367).This antibody is equivalent to Ser757 in mice.