Phospho-ULK1 (Ser758) Recombinant monoclonal antibody

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  WB analysis using 82776-8-RR

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Alpha Tubulin antibody (66031-1-Ig) as loading control.

• 

  WB analysis using 82776-8-RR

  Non-treated, phosphatase inhibitor treated and λ phosphatase treated PC-3 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Vinculin antibody (66305-1-Ig) as loading control.

Phospho-ULK1 (Ser758) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82776-8-RR

Clone No.240770A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

P ULK1 (Ser758), Phos ULK1 (Ser758), Phospho-ULK1 (Ser757), Phos-ULK1 (Ser758), P-ULK1 (Ser758)

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Tested Applications

• Positive WB detected in: IGF-1 treated MCF-7 cells, PC-3 cells, λ phosphatase treated PC-3 cells

This antibody is equivalent to Ser757 in mice.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82776-8-RR targets Phospho-ULK1 (Ser758) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: unc-51-like kinase 1 (C. elegans)
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75385
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367).This antibody is equivalent to Ser757 in mice.