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Phospho-Vimentin (Ser39) Recombinant monoclonal antibody, PBS Only (87714-1-PBS)

Filter: AllWBFC (Intra)

WB analysis of PANC-1 using 87714-1-RR (same clone as 87714-1-PBS)
PANC-1 cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.
WB analysis using 87714-1-RR (same clone as 87714-1-PBS)
Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.
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Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.
WB analysis using 87714-1-RR (same clone as 87714-1-PBS)
Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.
×
Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.
WB analysis using 87714-1-RR (same clone as 87714-1-PBS)
Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 87714-1-RR (same clone as 87714-1-PBS)
1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-Vimentin (Ser39) Recombinant monoclonal antibody (87714-1-RR, Clone:253259C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.
×
1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-Vimentin (Ser39) Recombinant monoclonal antibody (87714-1-RR, Clone:253259C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87714-1-PBS in a different storage buffer formulation.

Phospho-Vimentin (Ser39) Recombinant monoclonal antibody, PBS Only

Name: Phospho-Vimentin (Ser39) Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 87714-1-PBS

Phospho-Vimentin (Ser39) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 87714-1-PBS

Clone No.253259C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

P Vimentin (Ser39), Phos Vimentin (Ser39), Phos-Vimentin (Ser39), P-Vimentin (Ser39), VIM

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87714-1-PBS targets Phospho-Vimentin (Ser39) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	vimentin
Calculated Molecular Weight	54 kDa
Observed Molecular Weight	57 kDa
GenBank Accession Number	NM_003380
Gene Symbol	VIM
Gene ID (NCBI)	7431
ENSEMBL Gene ID	ENSG00000026025
RRID	AB_3745713
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08670
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Vimentin, a kind of intermediate filament protein III in mesenchymal cells, has become a highly researched topic around the world in recent years, as it holds complex biological functions and plays an important role in the epithelial-mesenchymal transition in the evolution of various tumors. Mesenchymal stem cells (MSCs) are widely studied for their application in cell therapy. The binding of AKT (tail region) to Vim (head region) results in Vim Ser39 phosphorylation enhancing the ability of Vim to induce motility and invasion while protecting Vim from caspase-induced proteolysis. (PMID: 33378968, PMID: 20856200, PMID: 31972217)