Phospho-Vimentin (Ser39) Recombinant monoclonal antibody

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• Phospho-Vimentin (Ser39) Recombinant monoclonal antibody (87714-1-RR)

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  WB analysis of PANC-1 using 87714-1-RR

  PANC-1 cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 87714-1-RR

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

• 

  WB analysis using 87714-1-RR

  Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

• 

  WB analysis using 87714-1-RR

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87714-1-RR (Phospho-Vimentin (Ser39) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 87714-1-RR

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-Vimentin (Ser39) Recombinant monoclonal antibody (87714-1-RR, Clone:253259C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-Vimentin (Ser39) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 87714-1-RR

Clone No.253259C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

P Vimentin (Ser39), Phos Vimentin (Ser39), Phos-Vimentin (Ser39), P-Vimentin (Ser39), VIM

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells, PANC-1 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HSC-T6 cells
• Positive FC (Intra) detected in: Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87714-1-RR targets Phospho-Vimentin (Ser39) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: vimentin
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_003380
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• ENSEMBL Gene ID: ENSG00000026025
• RRID: AB_3745713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08670
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, a kind of intermediate filament protein III in mesenchymal cells, has become a highly researched topic around the world in recent years, as it holds complex biological functions and plays an important role in the epithelial-mesenchymal transition in the evolution of various tumors. Mesenchymal stem cells (MSCs) are widely studied for their application in cell therapy. The binding of AKT (tail region) to Vim (head region) results in Vim Ser39 phosphorylation enhancing the ability of Vim to induce motility and invasion while protecting Vim from caspase-induced proteolysis. (PMID: 33378968, PMID: 20856200, PMID: 31972217)