CoraLite® Plus 647-conjugated Phospho-eEF2K (Ser366) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-eEF2K (Ser366) Recombinant monoclonal antibody (CL647-80351-3)

Phospho-eEF2K (Ser366) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-80351-3 Copied!

Clone No. 250361E10

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

eEF2K (Ser366), eEF2K(Ser366), p eEF2K, p eEF2K (Ser366), p-eEF2K

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  FC experiment of Jurkat using CL647-80351-3

  1X10^6 Jurkat cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Eef2k (Ser366) Recombinant Antibody (CL647-80351-3, Clone:250361E10)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: λ phosphatase treated Jurkat cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80351-3 targets Phospho-eEF2K (Ser366) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: eukaryotic elongation factor-2 kinase
• Calculated Molecular Weight: 725 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC032665
• Gene Symbol: EEF2K
• Gene ID (NCBI): 29904
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00418
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Eukaryotic Elongation Factor-2 Kinase (eEF2K) acts as a negative regulator of protein synthesis, translation, and cell growth. As a structurally unique member of the alpha-kinase family, eEF2K is essential to cell survival under stressful conditions, as it contributes to both cell viability and proliferation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is downregulated through the phosphorylation of multiple sites via mTOR signaling and upregulated via the AMPK pathway. In the S6K-mediated pathway, RPS6KB1 or p70S6K, can phosphorylate eEF2K on Ser-366, rendering it inactive(PMID: 11500364, PMID: 34532346). The calculated molecular weight of eEF2K is 82kDa, and probably due to phosphorylation modification, resulting in a larger molecular weight, around 100kDa.