Podocalyxin Monoclonal antibody, PBS Only

Podocalyxin Monoclonal Antibody for IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A3C7

Cat no : 68250-1-PBS

Synonyms

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60

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Validation Data Gallery

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  IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68250-1-Ig (same clone as 68250-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Podocalyxin (68250-1-Ig, Clone:4A3C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68250-1-PBS targets Podocalyxin in IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Podocalyxin fusion protein Ag12859
• Full Name: podocalyxin-like
• Calculated Molecular Weight: 526 aa, 55 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC093730
• Gene Symbol: PODXL
• Gene ID (NCBI): 5420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).