Mouse PON2 Recombinant monoclonal antibody, PBS Only (Capture)

• 

  WB analysis using 85126-4-RR (same clone as 85126-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85126-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85126-4-RR (same clone as 85126-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85126-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01843-1

  Sandwich ELISA standard curve of MP01843-1, Mouse Pon2 Recombinant Matched Antibody Pair - PBS only. 85126-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2741. 85126-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  Sample test of MP01843-1

  The mean Pon2 concentration was determined to be 344.03 ng/mL in mouse liver tissue extract based on a 3.70 mg/mL extract load, 812.45 ng/mL in rat liver tissue extract based on a 2.60 mg/mL extract load and 19.36 ng/mL in NIH/3T3 cell extract based on a 1.50 mg/mL extract load.

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  Cytometric bead array standard curve of MP01843-1

  Cytometric bead array standard curve of MP01843-1, MOUSE Pon2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85126-4-PBS. Detection antibody: 85126-2-PBS. Standard: Eg2741. Range: 1.563-200 ng/mL.

PON2 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85126-4-PBS

Clone No.242673H1

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

242673H1, A-esterase 2, Aromatic esterase 2, EC:3.1.1.81, PON 2

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Product Information

85126-4-PBS targets PON2 as part of a matched antibody pair:

MP01843-1: 85126-4-PBS capture and 85126-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2741

  Product name: Recombinant Mouse Pon2 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 30-354 aa of NM_183308.2

  Sequence: ASREVESVDLPNCHLIKGIETGAEDIDILPNGLAFFSVGLKFPGLHSFAPDKPGGILMMDLKDERPRALELRVSWGFDLASFNPHGISTFIDDDDTVYLFVVNHPQFKSTVEIFKFQEEENSLLHLKTIKHELLPSVNDIIAVGPTHFYATNDHYFSDPFLKYLETYLNLHWANVVYYSPEEVKLVAEGFDSANGINISPDKKYVYVADILAHEIHVLEKQPNMNLTQLKVLQLGTLVDNLSIDPSSGDIWVGCHPNGQRLFVYHPNHPPASEVLRIQNILSEKPSVTTVYINNGSVLQGSSVATIYDRKLLVGTLYQKALYCEL

• Full Name: paraoxonase 2
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: NM_183308.2
• Gene Symbol: Pon2
• Gene ID (NCBI): 330260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62086
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).