Progesterone Receptor Recombinant antibody

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  WB analysis of MCF-7 using 85112-4-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 85112-4-RR (PR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85112-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85112-4-RR (Progesterone Receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 85112-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 85112-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of T-47D using 85112-4-RR

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells, MCF-7 cells, MDA-MB-231 cells using PR antibody (85112-4-RR, Clone: 242686G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85112-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85112-4-RR against Human Progesterone Receptor were performed. The affinity constant is 0.587 nM.

Progesterone Receptor Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85112-4-RR

Clone No.242686G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PGR, NR3C3, PR, PRGR

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Tested Applications

• Positive WB detected in: MCF-7 cells, T-47D cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: T-47D cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85112-4-RR targets Progesterone Receptor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Progesterone Receptor fusion protein Ag22986
• Full Name: progesterone receptor
• Calculated Molecular Weight: 933 aa, 99 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: NM_000926
• Gene Symbol: Progesterone Receptor
• Gene ID (NCBI): 5241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06401
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.