PRX5 Monoclonal antibody

• Featured Product
• KD/KO Validated

PRX5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1B8C5

Cat no : 67599-1-Ig

Synonyms

ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, AOPP, B166, Liver tissue 2D page spot 71B, peroxiredoxin 5, Peroxiredoxin 5, mitochondrial, Peroxiredoxin V, Peroxisomal antioxidant enzyme, PLP, PMP20, PRDX5, PRDX6, Prx V, Prx5, PRXV, SBBI10, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI

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Validation Data Gallery

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  WB analysis using 67599-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 67599-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67599-1-Ig

  WB result of PRX5 antibody (67599-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRX5 transfected HepG2 cells.

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  IHC staining of human liver cancer using 67599-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67599-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67599-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRX5 antibody (67599-1-Ig, Clone: 1B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, LNCaP cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10400
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

67599-1-Ig targets PRX5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRX5 fusion protein Ag12262
• Full Name: peroxiredoxin 5
• Calculated Molecular Weight: 214 aa, 22 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC110983
• Gene Symbol: PRX5
• Gene ID (NCBI): 25824
• RRID: AB_2882805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
rat	WB,IF	Brain Res Bull; Peroxiredoxin-5 alleviates early brain injury after subarachnoid hemorrhage by reducing oxidative stress; Authors - Jinshuo Yang
human	WB,IP,IF	Int J Biol Macromol; Prebiotic stachyose inhibits PRDX5 activity and castration-resistant prostate cancer development; Authors - Rong Wang