GlnRS Polyclonal antibody

• 

  WB analysis using 12645-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12645-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12645-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12645-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12645-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 12645-1-AP

  IP result of anti-GlnRS (IP:12645-1-AP, 4ug; Detection:12645-1-AP 1:500) with PC-3 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 12645-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12645-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 12645-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GlnRS antibody (12645-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 12645-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GlnRS antibody (12645-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

GlnRS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12645-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

QARS, SYQ, QARS1, Glutaminyl-tRNA synthetase, Glutamine--tRNA ligase

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, NIH/3T3 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:1500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• RIP: See 1 publications below

Product Information

12645-1-AP targets GlnRS in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3375

  Product name: Recombinant human QARS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 362-717 aa of BC016634

  Sequence: VKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

• Full Name: glutaminyl-tRNA synthetase
• Calculated Molecular Weight: 775 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC016634
• Gene Symbol: GlnRS
• Gene ID (NCBI): 5859
• RRID: AB_2098676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47897
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Protein Cell; Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation; Authors - Kai-Hui Zhang
human	WB	Nat Cell Biol; Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.; Authors - Maria C Passarelli
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
human	WB	Nucleic Acids Res; Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.; Authors - Haissi Cui
human	WB,RIP	EMBO Rep; The tRNA-like small noncoding RNA mascRNA promotes global protein translation.; Authors - Xinping Lu ; KD Validated
human	WB	Protein Cell; Glutamine signaling specifically activates c-Myc and Mcl-1 to Facilitate cancer cell proliferation and survival; Authors - Meng Wang