Filter: AllIHCIF/ICC

IHC staining of human pancreas cancer using 55326-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55326-1-AP (QSER1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 55326-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55326-1-AP (QSER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 55326-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55326-1-AP (QSER1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 55326-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55326-1-AP (QSER1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 55326-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using QSER1 antibody (55326-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

QSER1 Polyclonal antibody

Name: QSER1 Polyclonal antibody
Brand: Proteintech
SKU: 55326-1-AP

QSER1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 55326-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

glutamine and serine rich 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55326-1-AP targets QSER1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	glutamine and serine rich 1
Calculated Molecular Weight	190 kDa
GenBank Accession Number	NM_001076786
Gene Symbol	QSER1
Gene ID (NCBI)	79832
RRID	AB_3743205
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q2KHR3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

QSER1, a recently characterized protein, whose activity has been tightly associated with the protection of DNA methylation sites in hESCs. QSER1 is widely up-regulated in multiple cancers, and its up-regulation correlates with poor clinic outcomes (PMID: 36371602). QSER1 depletion leads to NODAL de-repression and associates with increased RNA Polymerase II occupancy at YAP1bound enhancers, pointing to a QSER1 contribution maintaining low transcriptional activity on certain genomic regions (PMID: 33833093, 35210841).