Tested Applications
| Positive WB detected in | HeLa cells, C6 cells, MCF-7 cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue, MDCK cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11808-1-AP targets RAB10 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2473 Product name: Recombinant human RAB10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC000896 Sequence: MAKKTYDLLFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHANEDVERMLLGNKCDMDDKRVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC Predict reactive species |
| Full Name | RAB10, member RAS oncogene family |
| Calculated Molecular Weight | 200 aa, 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC000896 |
| Gene Symbol | RAB10 |
| Gene ID (NCBI) | 10890 |
| RRID | AB_2173442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61026 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB10 antibody 11808-1-AP | Download protocol |
| IHC protocol for RAB10 antibody 11808-1-AP | Download protocol |
| IP protocol for RAB10 antibody 11808-1-AP | Download protocol |
| WB protocol for RAB10 antibody 11808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB10 Polyclonal Antibody (Cat# 11808-1-AP) has 32 citations in journals including Cell Research, Nature Communications, PNAS, Current Biology, eLife, Development, Oncotarget, and Journal of Neuroscience. Research fields span neuroscience (axon development, synaptic function, neurodegeneration), oncology (nasopharyngeal, colorectal, hepatocellular, lung cancers), cell biology (vesicular trafficking, autophagy), immunology (TLR4 signaling), pharmacology, proteomics, cardiology, and reproductive biology. Cited applications include WB, IF, IP, CoIP, and IHC across human, mouse, rat, pig, and zebrafish.
| Species | Application | Title |
|---|---|---|
Cell Res MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development. | ||
Nat Commun Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development. | ||
J Exp Clin Cancer Res Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway. | ||
Sci Total Environ Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10 | ||
Proc Natl Acad Sci U S A Ras-related protein Rab10 facilitates TLR4 signaling by promoting replenishment of TLR4 onto the plasma membrane. | ||
Food Chem X Succinylation proteomic analysis identified differentially expressed succinylation sites affecting porcine muscle fiber type function. |
Reviews
What customers say
The single review for this product (Rab10 antibody) was for Western Blot at a 1:500 dilution on mouse brain lysate. The user rated it 4 out of 5, noting it works for detecting endogenous Rab10 but may require a higher concentration for optimal results. Overall, the feedback is positive, with the antibody performing adequately for its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nuo (Verified Customer) (11-07-2024) | It is okay to use it to probe endogenous Rab10 but it may need a higher concentration.
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