CoraLite® Plus 488-conjugated RAB10 Polyclonal antibody

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  IF Staining of NIH/3T3 using CL488-11808

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 RAB10 antibody (CL488-11808) at dilution of 1:200.

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  IF Staining of MCF-7 using CL488-11808

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 RAB10 antibody (CL488-11808) at dilution of 1:200.

• Featured Product
• KD/KO Validated

RAB10 Polyclonal Antibody for IF/ICC

Cat No. CL488-11808

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF/ICC

Synonyms

RAB10, Ras related protein Rab 10

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells, MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11808 targets RAB10 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2473

  Product name: Recombinant human RAB10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-200 aa of BC000896

  Sequence: MAKKTYDLLFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHANEDVERMLLGNKCDMDDKRVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC

• Full Name: RAB10, member RAS oncogene family
• Calculated Molecular Weight: 200 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000896
• Gene Symbol: RAB10
• Gene ID (NCBI): 10890
• RRID: AB_3672514
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61026
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.