RAB10 Recombinant monoclonal antibody

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  WB analysis using 86787-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86787-1-RR (RAB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86787-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using RAB10 antibody (86787-1-RR, Clone: 251744G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86787-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86787-1-RR against Human RAB10 were performed. The affinity constant is 3.91 nM.

RAB10 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86787-1-RR

Clone No.251744G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.6.5.2, Ras related protein Rab 10, Ras-related protein Rab-10

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HUVEC cells, C2C12 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86787-1-RR targets RAB10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag25830

  Product name: Recombinant human RAB10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 130-200 aa of BC000896

  Sequence: RVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC

• Full Name: RAB10, member RAS oncogene family
• Calculated Molecular Weight: 200 aa, 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC000896
• Gene Symbol: RAB10
• Gene ID (NCBI): 10890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61026
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB10 is a member of the RAB family of small GTPases, which are key regulators of vesicular trafficking. RAB proteins are cyclically controlled, requiring a GDP-GTP exchange that is facilitated by RAB guanine nucleotide exchange factors. Rab10 plays essential functional roles in development: mice are embryonic lethal when both alleles are deleted. In addition, RAB10 plays a role in maintenance and regulation of endoplasmic reticulum (ER) morphology. RAB10 function has also been linked to neuronal morphology and polarization, playing a critical role in axonal development and dendrite arborization.