RAB11A Monoclonal antibody, PBS Only

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  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67902-1-Ig (same clone as 67902-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67902-1-Ig (same clone as 67902-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67902-1-Ig (same clone as 67902-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67902-1-Ig (same clone as 67902-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A antibody (67902-1-Ig, Clone: 4A4C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67902-1-Ig (same clone as 67902-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RAB11A Monoclonal antibody (67902-1-Ig, Clone:4A4C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

RAB11A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 67902-1-PBS

Clone No.4A4C9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Rab 11, RAB11, RAB11A/B, 4A4C9, EC:3.6.5.2

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Product Information

67902-1-PBS targets RAB11A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8879

  Product name: Recombinant human RAB11A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-216 aa of BC013348

  Sequence: MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI

• Full Name: RAB11A, member RAS oncogene family
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC013348
• Gene Symbol: RAB11A
• Gene ID (NCBI): 8766
• RRID: AB_2918658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P62491
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).