RAB11A Monoclonal antibody

RAB11A Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4A4C9

Cat no : 67902-1-Ig

Synonyms

Rab 11, RAB11, RAB11A, Ras related protein Rab 11A, YL8

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Validation Data Gallery

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  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of mouse brain using 67902-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67902-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67902-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67902-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB11A antibody (67902-1-Ig, Clone: 4A4C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A431 cells, A549 cells, HeLa cells, pig brain tissue, rat brain tissue, rat cerebellum tissue, HepG2 cells, K-562 cells, rabbit brain tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67902-1-Ig targets RAB11A in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB11A fusion protein Ag8879
• Full Name: RAB11A, member RAS oncogene family
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC013348
• Gene Symbol: RAB11A
• Gene ID (NCBI): 8766
• RRID: AB_2918658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).