RAB11A/B Recombinant monoclonal antibody

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• RAB11A/B Recombinant monoclonal antibody (87237-1-RR)

• KD/KO Validated

RAB11A/B Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 87237-1-RR

Clone No.252492E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Rab 11, RAB11, RAB11A, EC:3.6.5.2, Rab-11

• 

  WB analysis using 87237-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87237-1-RR (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 87237-1-RR

  WB result of RAB11A/B antibody (87237-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A/B transfected A431 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse spleen using 87237-1-RR

  IP result of anti-RAB11A/B (IP:87237-1-RR, 4ug; Detection:87237-1-RR 1:3000) with mouse spleen tissue lysate 2000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87237-1-RR targets RAB11A/B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8742

  Product name: Recombinant human RAB11A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-216 aa of BC013348

  Sequence: MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI

• Full Name: RAB11A, member RAS oncogene family
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC013348
• Gene Symbol: RAB11A
• Gene ID (NCBI): 8766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62491
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).