RAB11FIP1 Polyclonal antibody

RAB11FIP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16778-1-AP

Synonyms

Rab-coupling protein, Rab11-FIP1, rab11 FIP1, Rab11 family-interacting protein 1, Rab coupling protein

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Validation Data Gallery

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  WB analysis of HepG2 using 16778-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 16778-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 16778-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16778-1-AP

  IP result of anti-RAB11FIP1 (IP:16778-1-AP, 4ug; Detection:16778-1-AP 1:600) with HepG2 cells lysate 3200ug.

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  IHC staining of human breast cancer using 16778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16778-1-AP

  Immunofluorescent analysis of HeLa cells using 16778-1-AP (RAB11FIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, COS-7 cells, SKOV-3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 2 publications below

Product Information

16778-1-AP targets RAB11FIP1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB11FIP1 fusion protein Ag10179
• Full Name: RAB11 family interacting protein 1 (class I)
• Calculated Molecular Weight: 649aa,71 kDa; 1283aa,137 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC077720
• Gene Symbol: RAB11FIP1
• Gene ID (NCBI): 80223
• RRID: AB_2878315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,RIP	Mol Cancer; CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein; Authors - Chi Hin Wong
human	WB	Brief Bioinform; mi-IsoNet: systems-scale microRNA landscape reveals rampant isoform-mediated gain of target interaction diversity and signaling specificity.; Authors - Li Guo
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
human	WB,IHC	Am J Cancer Res; In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer; Authors - Qiankun Zhu
rat	WB,IF	ACS Appl Mater Interfaces; Lithium Promotes Osteogenesis via Rab11a-Facilitated Exosomal Wnt10a Secretion and β-Catenin Signaling Activation; Authors - Changjun Chen