RAB11FIP2 Polyclonal antibody, PBS Only

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• RAB11FIP2 Polyclonal antibody, PBS Only (18136-1-PBS)

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  WB analysis using 18136-1-AP (same clone as 18136-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18136-1-AP (same clone as 18136-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 18136-1-AP (same clone as 18136-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18136-1-AP (same clone as 18136-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB11FIP2 antibody (18136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18136-1-AP (same clone as 18136-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using RAB11FIP2 antibody 18136-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RAB11FIP2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 18136-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NRip11, Rab11 family-interacting protein 2, Rab11 FIP2, Rab11-FIP2

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Product Information

18136-1-PBS targets RAB11FIP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12736

  Product name: Recombinant human RAB11FIP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-373 aa of BC075073

  Sequence: MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSD

• Full Name: RAB11 family interacting protein 2 (class I)
• Calculated Molecular Weight: 512 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC075073
• Gene Symbol: RAB11FIP2
• Gene ID (NCBI): 22841
• RRID: AB_10597110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L804
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RAB11FIP2, or RAB11 family interacting protein 2, is a protein that plays a crucial role in intracellular trafficking, particularly in the recycling of endocytic vesicles. It is involved in several cellular processes, including TRAM-dependent toll-like receptor 4 signaling pathway, phagocytosis, and positive regulation of GTPase activity. RAB11FIP2 is localized in endosomes, nucleoplasm, and phagocytic cups, and it is expressed ubiquitously in various tissues, with higher expression in endometrium and brain. RAB11FIP2 interacts with the actin-based motor myo5b and the small GTPase Rab11, which are key components in the regulation of vesicle tethering and transport along actin filaments. This interaction is particularly important for processes such as cell polarization, motility, and lumen formation in multicellular structures. .