RAB11FIP3 Polyclonal antibody

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  WB analysis of rat brain using 25843-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25843-1-AP (RAB11FIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25843-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25843-1-AP (RAB11FIP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25843-1-AP (RAB11FIP3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 25843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25843-1-AP (RAB11FIP3 antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

RAB11FIP3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25843-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

ARFO1, Arfophilin 1, Arfophilin-1, EF hands-containing Rab-interacting protein, Eferin

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 5 publications below

Product Information

25843-1-AP targets RAB11FIP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22937

  Product name: Recombinant human RAB11FIP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 407-756 aa of BC051360

  Sequence: CNGQLGCSDPAFLTPSPTKRLSSKKVARYLHQSGALTMEALEDPSPELMEGPEEDIADKVVFLERRVLELEKDTAATGEQHSRLRQENLQLVHRANALEEQLKEQELRACEMVLEETRRQKELLCKMEREKSIEIENLQTRLQQLDEENSELRSCTPCLKANIERLEEEKQKLLDEIESLTLRLSEEQENKRRMGDRLSHERHQFQRDKEATQELIEDLRKQLEHLQLLKLEAEQRRGRSSSMGLQEYHSRARESELEQEVRRLKQDNRNLKEQNEELNGQIITLSIQGAKSLFSTAFSESLAAEISSVSRDELMEAIQKQEEINFRLQDYIDRIIVAIMETNPSILEVK

• Full Name: RAB11 family interacting protein 3 (class II)
• Calculated Molecular Weight: 756 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC051360
• Gene Symbol: RAB11FIP3
• Gene ID (NCBI): 9727
• RRID: AB_2880264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75154
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Dev Cell; Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis.; Authors - Vijay Walia ; KD Validated
human	IF	J Cell Sci; Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.; Authors - Nicholas V G Iannantuono
human	IF	J Cell Sci; Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.; Authors - Nicholas V G Iannantuono
human	WB,IF	Cell Rep; Rab11-Rab8 cascade dynamics in primary cilia and membrane tubules; Authors - Ipsita Saha ; KD Validated
human,mouse	WB,IF	J Extracell Vesicles; Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells; Authors - Sara Busatto