RAB11FIP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB11FIP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25843-1-AP

Synonyms

ARFO1, Arfophilin 1, Eferin, FIP3 Rab11, KIAA0665, MU MB 17.148, Rab11 FIP3, RAB11FIP3

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Validation Data Gallery

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  WB analysis of rat brain using 25843-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25843-1-AP (RAB11FIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25843-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25843-1-AP (RAB11FIP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25843-1-AP (RAB11FIP3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25843-1-AP (RAB11FIP3 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 3 publications below

Product Information

The immunogen of 25843-1-AP is RAB11FIP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB11FIP3 fusion protein Ag22937
• Full Name: RAB11 family interacting protein 3 (class II)
• Calculated Molecular Weight: 756 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC051360
• Gene Symbol: RAB11FIP3
• Gene ID (NCBI): 9727
• RRID: AB_2880264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Dev Cell; Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis.; Authors - Vijay Walia ; KD Validated
human	IF	J Cell Sci; Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.; Authors - Nicholas V G Iannantuono
human	IF	J Cell Sci; Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.; Authors - Nicholas V G Iannantuono